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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in several tumors, which ends up in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two extra splice variants were claimed but detailed nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.
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This tutorial outlines even further probable optimization of cellular and immunoassay parameters to make certain the very best effects are...
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This guidebook outlines even more possible optimization of mobile and immunoassay parameters to make sure the very best final results are...
Antibodies bound to the blocking peptide not bind to your epitope over the goal protein. By evaluating the staining from the blocked antibody compared to the antibody by yourself, you can see which staining is precise.
Focus on Information Cyclin E belongs to the extremely conserved cyclin family, whose members are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins show distinct expression and degradation patterns which add to your temporal coordination of every mitotic function. Cyclin E varieties a fancy with and features being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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